Quality custom oligonucleotides for research and preclinical applications
Our preclinical/OEM manufacturing service provides academic and industry partners with an ISO 9001-certified facility, cost-effective and timely oligonucleotide materials for novel research (in vitro) thru preclinical use (in vivo). .
With an ISO 9001-certified manufacturing facility and a highly trained and dedicated custom oligonucleotide synthesis team, Dharmacon offers:
Are you interested in a preclinical/OEM custom service? To get started, please Request Pricing with our custom oligonucleotide synthesis form. For further assistance, please contact Technical Support
Purity analysis and characterization of a 42-mer crRNA oligonucleotide. The 42-mer RNA was synthesized using 2’-O-TBDMS chemistry at 120 µmol scale. After cleavage and deprotection, the RNA oligonucleotide was purified by anion-exchange chromatography. Pooled fractions were desalted by ultra-filtration followed by freeze drying to yield lyophilized RNA. (A) Analytical ion-pairing reverse phase UPLC trace showing 92% full-length product (FLP); (B) Analytical anion-exchange HPLC trace showing 89% full-length product (FLP); (C) Mass was analyzed with the LXQ Mass Spectrometer, confirming the correct mass.
Analysis of a 30-mer DNA oligonucleotide with final delivery yield of 1 g. (A) Analytical trace from ion pairing reverse phase UPLC showing 97% full length material. (B) Mass was analyzed by ESI-MS, results illustrate the correct mass.