On-demand custom siRNA screening libraries for ANY species
Enjoy algorithm-designed siRNA produced with a novel enzymatic method to support large functional genomics studies in non-human or mouse models. Whether you only want to screen 1000 key gene targets or a whole genome, we now have a solution for you! All that's required is species information and a gene list to get a targeted siRNA library for your model organism.
Large scale siRNA screening libraries are no longer limited to human and mouse! For researchers working with alternative species (dog, pig, rat, cow, mosquito, hamster, monkey, etc.), we have developed a novel enzymatic method to generate large arrayed libraries of algorithm-designed siRNAs for ANY species with an annotated genome!*
*Target species must have an annotated genome in either the RefSeq or Ensembl™ (Genome Research Limited) database for use as input to the SMARTselection siRNA design algorithm.
Would you like to learn more about Zoonome siRNA Libraries, or submit a request for a price quote? Due to their highly custom nature, all Zoonome libraries are sold by quote only. Complete the form below to have a product expert contact you. Note that orders can take up to 12 weeks to fulfill, so don't delay and inquire today!
The following species have been pre-validated to have an annotated genome database in Ensembl™ (Genome Research Limited) or RefSeq that is suitable for generation of genomewide siRNA designs for creation of a Zoonome siRNA library.
Just submit it, along with the other requested information on the Request Pricing form, and we will determine feasibility for you.
Enzymatic methods for siRNA production involve more steps than chemical synthesis, but are less expensive overall and result in a smaller quantity that is appropriate for a single end-user. Zoonome siRNA libraries utilize novel enzymatic methods to generate on-demand arrayed siRNA libraries for model species where synthetic libraries are not available.
Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells
Figure 1. CHO-K1 (Chinese hamster) cells were treated with z-siRNA pools targeting 16 genes and assessed for functional knockdown (upper graph) and cell viability (lower graph). All z-siRNA reagents achieved potent knockdown (>75%) while maintaining high cellular viability. Z-siRNAs at 25 nM were delivered with DharmaFECT 4, 0.5 ug per well and assayed 48 hr post-transfection.