• banner: Zoonome custom siRNA Libraries - Arrayed RNAi screening libraries for ANY species!  

    Zoonome Custom siRNA Libraries

    On-demand custom siRNA screening libraries for ANY species

    Enjoy algorithm-designed siRNA produced with a novel enzymatic method to support large functional genomics studies in non-human or mouse models. Whether you only want to screen 1000 key gene targets or a whole genome, we now have a solution for you! All that's required is species information and a gene list to get a targeted siRNA library for your model organism.

    Large scale siRNA screening libraries are no longer limited to human and mouse! For researchers working with alternative species (dog, pig, rat, cow, mosquito, hamster, monkey, etc.), we have developed a novel enzymatic method to generate large arrayed libraries of algorithm-designed siRNAs for ANY species with an annotated genome!*

    What are Zoonome siRNA libraries?

    • Utilize a novel enzymatic process for generation of functional, 21 mer siRNA duplexes for arrayed knockdown studies
    • On-demand plated collections of pooled siRNAs (4 algorithm-designed siRNAs in a single well) for RNAi screening studies in non-human or mouse cells
    • Minimum 1000 genes up to whole genome collections for any organism with an annotated genome
    • Expert siRNA design from the Dharmacon SMARTselection algorithm
    • Provided in 384-well plates (96-well upon request) for high-throughput studies

    *Target species must have an annotated genome in either the RefSeq or Ensembl™ (Genome Research Limited) database for use as input to the SMARTselection siRNA design algorithm.

    Would you like to learn more about Zoonome siRNA Libraries, or submit a request for a price quote? Due to their highly custom nature, all Zoonome libraries are sold by quote only. Complete the form below to have a product expert contact you. Note that orders can take up to 12 weeks to fulfill, so don't delay and inquire today!

    The following species have been pre-validated to have an annotated genome database in Ensembl™ (Genome Research Limited) or RefSeq that is suitable for generation of genomewide siRNA designs for creation of a Zoonome siRNA library.

    Your species of interest isn't on the list? No problem!

    Just submit it, along with the other requested information on the Request Pricing form, and we will determine feasibility for you.

    Mammals
    Bonobo Pan paniscus
    Cat Felis catus
    Chimpanzee Pan troglodytes
    Chinese Hamster Cricetulus griseus
    Cow Bos taurus
    Dog Canis familiaris
    Dolphin Tursiops truncatus
    Elephant Loxodonta africana
    Ferret Mustela putorius furo
    Goat Capra hircus
    Golden Hamster Mesocricetus auratus
    Gorilla Gorilla gorilla gorilla
    Guinea Pig Cavia porcellus
    Horse Equus caballus
    Marmoset Callithrix jacchus
    Megabat Pteropus vampyrus
    Microbat Myotis lucifugus
    Olive Baboon Papio anubis
    Opossum Monodelphis domestica
    Orangutan Pongo pymaeus abelii
    Panda Ailuropoda melanoleuca
    Pig Sus scrofa
    Platypus Ornithorhynchus anatinus
    Rabbit Oryctolagus cuniculus
    Rat Rattus norvegicus
    Rhesus Monkey Macaca mulatta
    Rhinoceros Ceratotherium simum simum
    Sheep Ovis aries
    Sloth Choloepus hoffmanni
    Squirrel Ictidomys tridecemlineatus
    Wallaby Macropus eugenii
    Birds
    Chicken Gallus gallus
    Duck Anas platyrhynchos
    Turkey Meleagris gallopavo
    Zebra Finch Taeniopygia guttata
    Fish
    Fugu Takifugu rubripes
    Medaka Oryzias latipes
    Nile Tilapia Oreochromis niloticus
    Stickleback Gasterosteus aculeatus
    Zebrafish Danio rerio
    Reptiles
    American Alligator Alligator mississippiensis
    Chinese Alligator Alligator sinensis
    Insects
    Armyworm Spodoptera frugiperda
    Fruitfly Drosophila melanogaster
    Monarch Butterfly Danaus plexippus
    Mosquito Aedes aegypti
    Amphibians
    Frog Xenopus tropicalis
    Other
    Sea Squirt Ciona intestinalis
    Purple Sea Urchin Strongylocentrotus purpuratus
    Roundworm Caenorhabditis elegans
    Yeast Saccharomyces cerevisiae
    workflow: Zoonome alternative methods

    Enzymatic methods for siRNA production involve more steps than chemical synthesis, but are less expensive overall and result in a smaller quantity that is appropriate for a single end-user. Zoonome siRNA libraries utilize novel enzymatic methods to generate on-demand arrayed siRNA libraries for model species where synthetic libraries are not available.

    Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells

    data figure: Functionality of Zoonome siRNA (z-siRNA) in CHO-K1 cells

    Figure 1. CHO-K1 (Chinese hamster) cells were treated with z-siRNA pools targeting 16 genes and assessed for functional knockdown (upper graph) and cell viability (lower graph). All z-siRNA reagents achieved potent knockdown (>75%) while maintaining high cellular viability. Z-siRNAs at 25 nM were delivered with DharmaFECT 4, 0.5 ug per well and assayed 48 hr post-transfection.