The RNAi consortium (TRC) is a collaborative effort based at the Broad Institute of MIT and Harvard, and includes six MIT and Harvard associated research institutions and five international life sciences organizations. We have partnered with the TRC to make the shRNA libraries, which are available to researchers worldwide, and to compile shRNA libraries for popular gene families and pathways.
The pLKO.1 reagents can be packaged into virus particles for use in transduction experiments. Specifically, the replication-incompetent viral particles can be efficiently produced using lentiviral packaging plasmids co-transfected in 293T packaging cells. The Trans-Lentiviral packaging system, which offers maximum biosafety and high titers, is recommended.
9 different shRNA subset libraries available for popular gene families and pathways
Already cloned into lentiviral vectors
Amenable to in vitro and in vivo applications, such as the creation of stable knockdowns
Lentiviral vector enables transduction of primary and non-dividing cell lines Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html
Human TRC gene families and pathways
Gene Ontology (GO) annotated collections targeting all genes in a particular gene family or biological pathway have been created from the human TRC lentiviral shRNA library. These arrayed sets contain all shRNA from the human TRC lentiviral shRNA library that target genes falling under specific GO terms. These collections will greatly simplify pathway analysis, target identification, and validation.
Available gene family and pathway sets
GO annotations are applied to genes by curators within the Gene Ontology Consortium and do not originate from us. Consult the Gene Ontology website for details.
Citing the library in Scientific Publications
In scientific publications, the libraries should be referred to as TRC-Hs1.0 (Human) and TRC-Mm1.0 (Mouse). Individual clones are uniquely identified by their TRC ID number (eg. TRCN0000014783).