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    RTF siRNA Libraries

    Human and mouse SMARTpool siRNA libraries in a novel pre-dispensed format to enable RNAi screening without the need for high-throughput automation.

    Reverse Transfection Format (RTF) siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

    RTF SMARTpool siRNA Libraries are ideal for the lab that wants to screen with siRNA, but doesn't have access to high-throughput automation. SMARTpool siRNA and validated control reagents are pre-dispensed at assay-ready amounts in replicate 96-well cell culture plates. No special handling is required prior to use. Simply resuspend the siRNA and add your cells.

    Benefits

    • Each library is supplied as six replicate plate sets containing 6.25 nmol of siRNA per well; sufficient to run two screens in triplicate (additional plate sets available)
    • RTF Optimization Plates are available to optimize transfection, controls, and cell density for your assay prior to beginning your screen
    • RTF libraries are available in clear-bottom plates with clear, black, or white-walled wells to support assays involving fluorescent or luminescent detection methods

    Click on a collection ✔ to view availability and ordering information for that RTF siRNA library.

    If you have a gene list of your own not reflected in these predefined collections, click here to Submit a Quote Request for a custom siRNA library.

    Which siRNA is right for you?

    Dharmacon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.

    Cost-effective, efficient silencing High specificity for reduced off-targets + efficient silencing Highly specific knockdown of long noncoding RNA (lncRNA) Target silencing in difficult-to-transfect cells
    siGENOME siRNA ON‑TARGETplus siRNA Lincode siRNA Accell siRNA
    Pre-designed for Human, Mouse and Rat protein-coding genes
    Pre-designed for Human and Mouse long noncoding RNA (lncRNA)
    Recommended for transfectable mammalian cells in culture
    Recommended for neuronal, suspension, primary and other difficult-to-transfect cells
    Recommended transfection reagent DharmaFECT transfection reagent DharmaFECT transfection reagent DharmaFECT transfection reagent None required
    Available as SMARTpool reagent
    Available as four individual siRNAs
    Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs
    Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading
    Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
    Modifications to facilitate cellular uptake without separate transfection reagents
    Stabilizing modifications to prevent nuclease-mediated degradation
    Sequence information provided with purchase

    Resources for RNAi Screening Libraries