Mouse ON-TARGETplus RTF - Cell Cycle Regulation

The only ready-to-use siRNA screening libraries available


Ready-to-use arrayed siRNA library in single-use plate sets. Just rehydrate, and add cells for RNAi screening of mouse cell cycle genes. Optimization plates available.

The Mouse ON-TARGETplus RTF Cell Cycle Regulation siRNA Library includes siRNA reagents targeting genes whose functions are crucial for controlling cell division. Many of the targets in this array play pivotal roles during specific phases of the cell cycle. Of particular importance to the progression of the cell cycle are the cyclin-dependent kinases, or CDKs. Other protein families known to regulate the cell cycle include the members of the CIP/KIP family and the INK4 family of cell cycle inhibitors, members of the retinoblastoma protein family, and DNA replication factors, such as the cell division cycle proteins (CDCs).

RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate and add cells. This unique pre-plated format reduces hands-on time for faster screening results.

ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.

Highlights

  • Six ready-to-use 96-well plate sets provided for two triplicate screens
  • Pre-plated, validated RNAi controls included
  • No aliquoting necessary - just resuspend, and add cells
  • siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
  • Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Well Pre-plated control Control Catalog No.
A1 ON-TARGETplus Non-targeting siRNA #1 D-001810-01
B1 ON-TARGETplus Non-targeting siRNA #2 D-001810-02
C1 ON-TARGETplus Non-targeting siRNA #3 D-001810-03
D1 ON-TARGETplus Non-targeting siRNA #4 D-001810-04
E1 ON-TARGETplus Non-targeting pool D-001810-10
F1 ON-TARGETplus Mouse Cyclophilin B control pool D-001820-20

Experimental considerations

DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates

DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.

Gene Targets

For a complete list of target genes in this siRNA Library, please contact Technical Support.

  
HazardousYes
Shipping ConditionAmbient
Stability at Recommended Storage ConditionsAt least 12 months
Storage Condition-20 C

Our siRNA knockdown guarantee

siGENOME and ON-TARGETplus siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100 nM siRNA).

Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25 nM working concentration.

Plate layout of ON-TARGETplus RTF siRNA Libraries

Plate layout of ON-TARGETplus RTF siRNA Libraries

Plate layout of ON-TARGETplus RTF siRNA Libraries

Figure 1. | Validated control siRNAs and pools are pre-dispensed into column 1 of each RTF Library plate, providing a consistent baseline for screening and assay efficiency.


Reverse Transfection Format is highly effective across cell lines

Reverse Transfection Format is highly effective across cell lines

Reverse Transfection Format is highly effective across cell lines

Figure 2. | Reverse transfection Format was used to assess control gene silencing (Cyclophilin B; blue bars) and viability (yellow dots) across eight cell lines under optimized conditions. In all cases, effective target gene knockdown was achieved with low cytotoxicity.


Simple four-step protocol for screening with RTF SMARTpool siRNA Libraries

Simple four-step protocol for screening with RTF SMARTpool siRNA Libraries

Simple four-step protocol for screening with RTF SMARTpool siRNA Libraries

Figure 3.


References

  1. Publications using RTF Libraries

    T. Sorkina, M. Miranda, K.R. Dionne, B.R. Hoover, N.R. Zahniser, A. Sorkin, RNA interference screen reveals an essential role of Nedd4-2 in dopamine transporter ubiquitination and endocytosis. J Neurosci. 26(31), 8195-205 (2006).

  2. P. Monteiro, D. Gilot, E. Le Ferrec, C. Rauch, D. Lagadic-Gossmann, O. Fardel, Dioxin-mediated up-regulation of aryl hydrocarbon receptor target genes is dependent on the calcium/calmodulin/CaMKIalpha pathway. Mol Pharmacol. 73(3), 769-77 (Epub 18 December 2007, March 2008).
  3. A. A. Kolokoltsov, D. Deniger, E. H. Fleming, N.J. Roberts Jr, J. M. Karpilow, R. A. Davey, Small interfering RNA profiling reveals key role of clathrin-mediated endocytosis and early endosome formation for infection by respiratory syncytial virus. J Virol. 81(14), 7786-800 (Epub 9 May 2007, July 2007).
  4. K. M. Hussain, K. L. Leong, M. M. Ng, J. J. Chu, The essential role of clathrin-mediated endocytosis in the infectious entry of human enterovirus 71. J Biol Chem. 286(1), 309-321 (Epub 18 October 2010, 7 January 2011).

  5. General Screening References

    B. D. Parsons, A. Schindler, D.H. Evans, E. Foley, A direct phenotypic comparison of siRNA pools and multiple individual duplexes in a functional assay. PLoS One. 4(12), e8471 (2009).

  6. M. Jiang, R. Instrell, B. Saunders, H. Berven, M. Howell, Tales from an academic RNAi screening facility; FAQs. Brief Funct. Genomics. 10(4), 227-237 (2011). [doi: 10.1093/bfgp/elr016]