The Human siGENOME RTF Ubiquitin Conjugation Subset 1 contains SMARTpool siRNA reagents targeting Cullin, E1, E2 and HECT E3 Ligases. Assessment of effects of E1 and E2 enzymes will provide a reasonable idea as to whether components of the ubiquitin system are involved. Also included are members of the HECT family of E3 ligases, which differ from the large majority of known E3s (RING finger and RING finger-like) in functioning as catalytic intermediates in ubiquitin conjugation.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
siGENOME siRNAs are designed with the proprietary SMARTselection design algorithm for high-efficiency, guaranteed silencing. They also incorporate rational strand bias with application of ON-TARGET modifications to optimize antisense strand loading into RISC for effective target knockdown.
Six ready-to-use 96-well plate sets provided for two triplicate screens
Pre-plated, validated RNAi controls included
No aliquoting necessary, just resuspend and add cells
SMARTpool siRNA reagents provided in clear plates at 6.25 pmol per well (50 nM final screening concentration)
Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Control Catalog No.|
siGENOME Non-targeting siRNA #2||
siGENOME Non-targeting siRNA #3||
siGENOME Non-targeting siRNA #4||
siGENOME Non-targeting siRNA #5||
siGENOME Non-targeting pool #2||
siGENOME Cyclophilin B control pool (H, M, R) Control siRNA||
DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
For a complete list of target genes in this siRNA Library, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
For a thorough investigation of the ubiquitin pathway, you may also consider these additional siRNA libraries: