The Human ON-TARGETplus RTF Deubiquitinating Enzyme siRNA library targets members of the deubiquitinating enzyme group, whose role is to remove ubiquitin. Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction.
RTF siRNA libraries are provided as multiple single-use plate sets – just rehydrate, and add cells. This unique pre-plated format reduces hands-on time for faster screening results.
ON-TARGETplus modifications combine sense strand inactivation with a novel seed-region modification. This patented siRNA approach is the best strategy to prevent off-target effects caused by both the sense and antisense strands while maintaining high silencing potency.
Six ready-to-use 96-well plate sets provided for two triplicate screens
Pre-plated, validated RNAi controls included
No aliquoting necessary,just resuspend, and add cells
siRNA reagents provided in clear plates at 6.25pmol per well (50nM final screening concentration)
Black or white clear-bottom plates available to support assays involving fluorescent or luminescent detection
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
Control Catalog No.|
ON-TARGETplus Non-targeting siRNA #1||
ON-TARGETplus Non-targeting siRNA #2||
ON-TARGETplus Non-targeting siRNA #3||
ON-TARGETplus Non-targeting siRNA #4||
ON-TARGETplus Non-targeting pool||
ON-TARGETplus Human Cyclophilin B control pool||
DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
For a complete list of target genes in this siRNA Library, please email Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
For a thorough investigation of the ubiquitin pathway, you may also consider our three Ubiquitin Conjugation libraries: