shMIMIC Lentiviral microRNAs were developed using a unique and proprietary design strategy resulting in highly functional microRNA mimics. shMIMIC lentiviral microRNA designs are created for the robust over-expression of each mature human, mouse and rat microRNA in the miRBase database while minimizing the effects of the star (*), or passenger, strand. Further, shMIMIC microRNAs are provided as glycerol stocks or purified high-titer lentiviral particles for delivery into biologically relevant cells and creation of stably integrated, microRNA-expressing cell lines.
Patented expression scaffold for consistent and correct processing of the mature microRNA
Your choice of four different constitutive promoters to optimize microRNA expression in your cells
Design modifications promote preferential loading of the mature microRNA into RISC and robust function
High-titer, concentrated lentiviral particles permits delivery into cells refractory to lipid-based delivery
Expression of TurboGFP or Turbo RFP (Evrogen, Moscow) allows visualization of transduction efficiency; a no-reporter format is also available
Glycerol stock format can be used to grow and isolate the lentiviral plasmid for direct transfection or packaging into lentiviral particles
Integrated over-expression of mature microRNA in biologically relevant cell lines advances the long-term study of microRNA-induced gene target modulation and phenotypic effects. microRNA-induced down-regulation of target genes is often subtle, and phenotypes difficult to discern. To ascertain the function of a microRNA, over-expression must be robust and reproducible. Thus, careful attention must be paid to the promoter driving the expression of the microRNA vector.
The SMARTchoice platform allows you to customize your shMIMIC microRNA with the promoter and reporter combination that is most suitable for your cells and assays.
Don't know which promoter is most active in your cells? Use the SMARTchoice Promoter Selection Plate to easily assess which promoter is optimal and most active in your cells of interest before purchasing specific shMIMIC lentiviral microRNAs (click to learn more about the SMARTchoice Promoter Selection Plate).
Lentiviral Particle Shipping and Storage Information
shMIMIC lentiviral microRNAs are made-to-order with your choice of promoter and reporter and require an estimated 3- to 6-week manufacturing time. Additional transit time should be expected for international shipment to account for customs and regulatory clearances and overseas transport.
shMIMIC lentiviral microRNAs are shipped on dry ice for overnight domestic delivery or priority international delivery outside of the USA. shMIMIC lentiviral particles can be stored frozen at -80ºC for 6 to 12 months without a significant loss in titer. Glycerol stocks can be stored at -80ºC indefinitely.
Lentiviral Particle Product Safety Level Information
This Lentiviral Particle Product Safety Level Information constitutes Product Documentation according to clause 1 of the Product Terms and Conditions.
The Products are solely for internal research use (as set forth in the Product Terms and Conditions) in laboratories where the required containment measures stated in applicable laws and regulations are met. Products may not be used for diagnostic, therapeutic or other commercial purposes and may not to be administered to humans for any purpose or to animals for therapeutic purposes. The Products are replication-incompetent, self-inactivating (SIN) and non-pathogenic (do not cause infectious human disease).
Note - Any investigator who purchases our lentiviral particle products is responsible for consulting with their institution’s health and biosafety personnel for specific guidelines on the handling of lentiviral vector particles. Furthermore, each investigator is fully responsible for obtaining the required permissions for research using and the acceptance of replication-incompetent SIN lentiviral vectors and replication-defective lentiviral particles into their local jurisdiction and institution.
For questions concerning the design or production of the products, please contact Technical support.