Up-regulate or suppress endogenous mature miRNA (miRNA is an abbreviation of microRNA) function with rationally designed synthetic and expressed miRNA modulation reagents.
Dharmacon miRNA reagents enable short- and long-term modulation of specific
miRNAs using a combination of patented technologies. These include chemical and structural enhancements on synthetic reagents and customizable and highly functional design elements on lentiviral reagents.
microRNAs are noncoding RNAs that mediate post-transcriptional gene regulation through a mechanism that involves RISC-mediated binding to complementary sequences largely in the 3’ UTR of mammalian target genes. microRNAs bind to targeted messenger RNAs (mRNAs) via imperfect sequence complementarity resulting largely in mRNA destabilization and translational attenuation. Because microRNA targeting of transcripts is directed by partial sequence complementarity, each microRNA can potentially regulate hundreds of genes.
Modulation of microRNA levels in both gain- and loss-of function experiments are a fundamental way to understand not only microRNA function, but also the pathways in which they may be involved.
miRIDIAN microRNA mimics are chemically synthesized synthetic double-stranded RNA molecules which are intended to mimic the endogenous duplex resulting from Dicer processing. One strand represents the mature microRNA as annotated in the miRBase database release 21.0 (www.mirbase.org). Use of the proprietary ON-Target modification ensures that only the desired mature strand is loaded into RISC. miRIDIAN microRNA mimics require delivery into cells using methods similar to those for siRNA (transfection reagents, electroporation, etc.). Individual reagents are available for every microRNA in human, mouse, and rat; and predefined screening libraries are available for human and mouse. Custom microRNAs can be ordered through our
custom microRNA web tool to allow more flexibility in species availability, design criteria and adding chemical modifications. Custom rearrays can also be generated and ordered through use of the Cherry-pick Library Plater.
shMIMIC Lentiviral microRNA and shMIMIC Lentiviral Inducible microRNA constructs are lentiviral vectors which encode native microRNA sequences to achieve exogenously introduced microRNA expression. Based on the patented SMARTvector Lentiviral shRNA platform, they are optimized for highly functional expression and to ensure strand bias so as to only load the specific targeting strand representing the mature microRNA sequence. With the added flexibility of the SMARTchoice promoter and reporter options, constructs can be ordered in numerous configurations ensuing optimal experimental results. Individual constructs are available as high-titer purified lentiviral particles for all human, mouse, and rat microRNAs in the miRbase database release 21.0; pooled screening libraries are also available for human and mouse in both constitutive and inducible formats. Custom pooled screening libraries can also be ordered here.
Introduction of microRNA inhibitors into a biological system results in a loss-of-function assay with a predicted decrease in endogenous microRNA function. miRIDIAN microRNA Hairpin Inhibitors are synthetic single-stranded RNA molecules designed to inhibit endogenous microRNA activity. They are the newest generation proprietary, synthetic inhibitors. The patent-pending molecule combines chemical modifications and completely novel secondary structure motif giving enhanced potency and longevity. This allows for multiplexed microRNA inhibition at very low nanomolar concentrations and with minimal toxicity. Individual reagents are available for every mature microRNA in human, mouse, and rat n the miRBase database release 21.0; and predefined inhibitor screening libraries are available for human and mouse. Custom rearrays can also be created and ordered through use of the Cherry-pick Library Plater.
Modify an existing microRNA mimic or inhibitor, target a novel microRNA, or request an Accell microRNA Inhibitor or Stabilized Mimic for special applications.
Order miRIDIAN microRNA mimics for gain-of-function studies. Use common miRBase identifiers to search for microRNAs in human, mouse and rat.
Order microRNA Hairpin Inhibitors to suppress microRNA activity. Use common miRBase identifiers to search for microRNAs in human, mouse and rat.
Expressed lentiviral microRNAs multiple promoter and reporter options for expression of mature microRNAs; available as high-titer lentiviral particles and glycerol stocks.
The industry's most innovative mature microRNA expression system combined with latest generation of powerful Tet-inducible technology; available as high-titer lentiviral particles and glycerol stocks
A complete portfolio of positive and negative experimental controls to optimize conditions and design relevant microRNA modulation experiments.
Validated miRIDIAN microRNA Mimics designed to target PPIB in human, mouse, and rat.
Validated miRIDAN microRNA Mimics designed to target GAPD in human, mouse and rat
A validated miRIDIAN microRNA Mimic designed to target Aldolase A in human, mouse, and rat
Differentiate between specific and non-specific effects with a negative control mimic
Visualize delivery and optimize transfection conditions with a fluorescently labeled mimic control
A validated microRNA inhibitor designed to target miR-16 in human, mouse and rat
Differentiate between specific and non-specific effects with a negative control inhibitor
Visualize delivery and optimize transfection conditions with a fluorescently labeled Hairpin Inhibitor control
Positive and negative constitutive shRNA controls with choice of seven promoters and three reporter options.
Inducible expression of positive and negative controls with your choice of four promoters and two reporters.
Mimic and Hairpin Inhibitor libraries are available for human, mouse, and rat microRNAs. Provided in 96-well plates for high-throughput phenotypic screening.
The miRIDIAN microRNA Mimic Library is a complete collection of microRNA mimics arrayed in 96- or 384-well plates to allow high-throughput phenotypic screening applications in human, mouse, or rat.
The miRIDIAN microRNA Hairpin Inhibitor Library is a complete collection of microRNA inhibitors arrayed in 96-well plates to allow high-throughput phenotypic screening applications in human, mouse, or rat.
Perform functional screening of hundreds or thousands of microRNAs without high-throughput automation.
Tight control of microRNA expression for powerful functional screening of hundreds or thousands of microRNAs.
Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.