siSTABLE GAPD Control siRNA is a validated positive control, guaranteed to silence GAPD in mouse cells. This control siRNA is chemically modified to significantly extend siRNA stability and is recommended for use as a positive control in experiments using siSTABLE modified siRNA against a target gene or where increased stability of the control is desired.
Also known as glyceraldehyde-3-phosphate dehydrogenase or GAPDH, GAPD is an important enzyme in carbohydrate metabolism that is well conserved across the animal kingdom. This gene is abundantly expressed in most cells, and because it is non-essential, knockdown of the corresponding mRNA does not affect cell viability. Targets accession number NM_001001303.
The siSTABLE modification pattern dramatically extends the half-life of siRNA in the presence of 100% human serum. Unmodified siRNA degrades almost immediately while siSTABLE siRNA maintains integrity for up to 5 days.
S. Cabodi et al., p130Cas is an essential transducer element in ErbB2 transformation. FASEB J. 24(10), 3796-3808 (October 2010).