Lincode siRNAs are modified with a proprietary dual-strand modification that improves siRNA functionality. Additionally, off-targets are reduced due to:
- Inactivation of passenger strand activity; driving preferential loading of the guide strand into RISC
- Novel seed region modifications for disruption of microRNA-like off-targets
Knockdown of endogenous GAS5 in HeLa cells and hNDF cells by GAS5 Control siRNA and GAS5 Control Pool. All siRNAs were used at 25 nM. Remaining lncRNA was detected by corresponding Solaris qPCR lncRNA expression assay. Knockdown was normalized to Non-targeting control siRNA. Viability was measured by resazurin assay and normalized to Untreated cells.