The SMARTvector Lentiviral shRNA experimental workflow begins with using the SMARTchoice Promoter Selection Plate to identify the most active vector configuration in your cells of interest simply by assessing TurboGFP fluorescence intensity in transduced cells. Then, order gene-specific shRNAs and matched-vector positive and negative controls with your choice of RNA polymerase II promoter and reporter. Transduce cells with SMARTvector Lentiviral shRNAs targeting genes of interest, always including matched positive and negative RNAi controls for rigorous data interpretation. All SMARTvector Lentiviral shRNAs and RNAi controls are provided as high-titer, purified and concentrated lentiviral particles for immediate transduction.
Figure 1. SMARTvector Lentiviral shRNA Controls: Non-Targeting Control and GAPD. K562, HUVEC, and SH-SY5Y cells were transduced at three MOIs and assessed for mRNA knockdown 72 hours post-transduction using a branched DNA assay (Panomics, Inc.)