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    Tools and Calculators

    Online tools for checking specificity of CRISPR target sequences, analyzing primers, identifying microRNA seed matches and performing other useful calculations.

    Gene editing

    • CRISPR Design Tool

      Generate custom synthetic crRNA sequences for use with the Edit-R Gene Engineering System and swiftly be on your way to gene knockouts!

    • CRISPR specificity analysis

      A fast, flexible specificity evaluator for CRISPR target sites.

    • Edit-R HDR Donor Designer

      Quickly and easily design and order a donor template for precise CRISPR-Cas9 gene editing!

    Primer design and analysis

    • Tm calculator

      Analyzes the Tm, MW and extinction coefficient of a primer, and gives dilution instructions.

    • Multiple primer analyzer

      Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.

    siRNA seed locator

    • siRNA seed locator

      This tool is available in support of efforts to develop intelligent and effective tools for selecting viable siRNA candidates and advancing the rational design of such.

    Biochemical conversions

    RNAi screening


    • qPCR Efficiency Calculator

      This calculator gives the amplification efficiency of a qPCR reaction based on the slope of the standard curve.

    BioIT Beta Tools

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