Every year since 2007, The Scientist Magazine gets hundreds (thousands?) of nominations for their Top 10 Innovations Award. Their goal is to recognize cutting-edge technologies that have the potential to dramatically change, and improve, life science research. Over the years, this recognition has spanned instruments to reagent kits and everything in between.
One of the 10 awards this year went to Edit-R™ Fluorescent Cas9 nuclease mRNA – a DNA-free source of Cas9 for CRISPR gene editing that co-expresses either mKate2 or EGFP. This enables researchers to perform genome engineering experiments in a completely DNA-free manner while still retaining the ability to enrich for transfected, and therefore edited, cells in a population.
Horizon Discovery and Dharmacon products have been fortunate to be recognized for our ongoing dedication to continued innovations and improvements for the research community.
A demonstration of the use of Edit-R Fluorescent Cas9 Nuclease mRNA and synthetic guide RNAs for enriching knockout and knock-in gene edited cells.
Purified Cas9 Nuclease mRNA for co-transfection with synthetic guide RNA for a completely DNA-free genome engineering system
A quick protocol for transfecting Edit-R Cas9 mRNA, synthetic guide RNA, and HDR donor template into mammalian cells using DharmaFECT Duo