The Dharmacon website will be undergoing scheduled maintenance starting Monday, November 19th at 9:00 a.m. (MST) and continuing until Tuesday, November 20th until 6:00 p.m. (MST). During this time period, there may be intermittent outages of the website. We thank you for your patience as we complete this necessary maintenance. If you need assistance, please reach out to our
customer support team
Home | Resources | FAQs
Will seed sibling controls always correctly identify phenotypes due to off-target effects?
No single approach should be relied upon to answer the important question of whether a hit in a siRNA screen is real or not. However, if there are multiple seed siblings that give a phenotype and do not silence the target gene of the experimental siRNA, it is highly likely to be due to an off-target. Similarly, the lack of phenotype with seed siblings will not guarantee that a hit is real, but it certainly will raise the confidence level of that siRNA/gene target as a real hit.