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Why are my wild-type yeast strains growing on kanamycin?
Ammonium sulfate will inactivate G418. Typically, kanamycin-resistant cells are selected on rich, yeast media [such as YPD] containing Geneticin Sulfate [G418 200ug/ml final]. High salt concentrations, primarily based on the inclusion of ammonium sulfate in the media, reduce the efficacy of G418 and therefore, G418-sensitive wild-type cells will grow on media containing G418 with ammonium sulfate as the nitrogen source.