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Why does the pCMV-SPORT6 vector map that Dharmacon shows differ from what Invitrogen shows?
The reason that the orientation of what is shown on the pCMV-SPORT6 vector map differs between Invitrogen and Dharmacon is because Invitrogen shows the minus (-) strand of the plasmid whereas Dharmacon shows the plus (+) strand. The reason that Dharmacon shows the pCMV-SPORT6 vector map this way is so that the promoter and cloning sites can visualized from left to right with respect to the way that most people view vector maps and sequences.