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Why are there ade1 and ade2 mutations in HA-tagged yeast?
The yeast mutations ade1 and ade2 result in the accumulation of a red pigment in the cells. Adenine sulfate is used to inhibit reversion of the ade1 and ade2 mutations. It doesn't have to be added for growth, but if you don't want the strain to revert (the parent strain for these has an ade2 mutation) you have to add adenine sulfate. Addition of adenine sulfate to the media will result in white colonies that still have the ade mutation.
You can refer to the following link for more information: http://www.phys.ksu.edu/gene/f_1.html