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What stock concentration is recommended for pre-designed siRNAs?
Pre-designed siRNAs are shipped as a dried pellet and are ready for resuspension in an appropriately buffered, RNase-free solution. We recommend stock concentrations between 20 - 100 uM, depending upon the initial quantity of siRNA supplied. For example, a 5 nmol quantity resuspended in 250 uL of buffer will result in a 20 uM stock solution. Alternatively, a 50 nmol quantity resuspended in 1 mL of buffer will result in a 50 uM stock solution. Please reference the FAQ under Calculations for information about how to calculate a specific stock concentration.