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What are the sequencing primers for the Yeast ORF clones in the BG1805 vector?
There is a T7 primer site located outside the fusion tag at the C-term. There is a T3 primer site located outside of the Gal1 promoter at the N-term. BG1805 primers, forward (5'-CATTTTCGGTTTGTATTACTTCTTATTC-3') and reverse (5'-GGACCTTGAAAAAGAACTTC-3')
Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky, EM, Snyder M, Grayhack EJ (2005). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes and Development19, 28162826.