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What quality control is performed on SMARTchoice Lentiviral shRNA Particles?
SMARTvector 2.0 shRNA constructs undergo multiple levels of analysis throughout production to ensure the quality of the final product. Restriction enzyme analysis and sequencing are performed on all viral vector constructs to ensure integrity of the lentiviral vector backbone and of the targeting sequence, respectively. Viral packaging cells are carefully monitored for bacterial and/or fungal contaminants. Following packaging, viral particles are titered by p24 ELISA. The p24 titers are adjusted to a functional titer using reference control viral particles titered by flow cytometry to count GFP-positive cells.