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What is the non-silencing control hairpin sequence?
The non-silencing control hairpin sequence is as follows:
22mer sense: ATCTCGCTTGGGCGAGAGTAAG
22mer antisense: CTTACTCTCGCCCAAGCGAGAG
This sequence does not match any known mammalian genes (had at least 3 or more mismatches against any gene as determined via nucleotide alignment/BLAST of 22mer sense sequence).
This is the non-silencing shRNAmir hairpin sequence found in the pSM2, pSMP, pGIPZ, pTRIPZ and pLemiR non-silencing controls.