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What is the formula for spectrophotometric quantitation of RNA?
To quantify RNA, the investigator should use Beer's Law: Absorbance (260 nm) = (epsilon)(concentration)(path length in cm), where epsilon is the molar extinction coefficient (provided on the Product Transfer Form supplied with the order). When solved for the unknown, the equation becomes:
Concentration = (Absorbance, 260 nm) / [(epsilon)(path length in cm)]. When a standard 10 mm cuvette is used, the path length variable in this equation is 1. If a different size of cuvette is used, e.g., a 2 mm microcuvette, then the path length variable is 0.2.