What cDNA or ORF clone verification methods should be taken before and after purchase?
The plate-row-column coordinates, DNA sequences, and annotations of our cDNA and ORF clones originate from the supplier and have not been independently verified by Dharmacon. We do, however, curate and act upon customer reported problems with clones. We therefore strongly recommend the following routine precautions:
1. Prior to purchase, the customer should analyze the GenBank sequence for the clone of interest using BLAST or other bioinformatics tools.
2. After purchase, the customer should end-sequence the clone and BLAST the result against the GenBank sequence.