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What do all the columns of the datasheet for the Yeast GST-tagged collection mean?
To address several questions regarding the datafile for the Yeast GST-tagged collection we have listed some of the most common questions and answers:
1) What does the column E represent?
Column D is “Gene Transformed”. If the source lab was unable to generate a yeast transformant because they couldn't retrieve any plasmid DNA from the original collection, then Column D will have the word BLANK and column E will have the gene name that they attempted to transform.
2) What is the difference among columns D, E and F?
Column D is the Gene name that was transformed. If it says BLANK, the source lab was unable to generate a yeast transformant because they couldn't retrieve any plasmid DNA from the original collection, and column E will have the gene name that they attempted to transform. Column F is a complete list of all of the yeast genes that were attempted for this collection. (Column D and E combined).
3) What do you mean by "empty vector" in columns D and F?
These wells should contain the empty pEGH Yeast GST fusion plasmid map-we have not verified or sequences this vector in house, but we believe it to be ~10,000bp.
5) What do you mean by N, X, Y and cells with no data in column G (Sequence
Y under Sequence ID column means yes - correct sequence ID.
N = no - sequence identity was incorrect and I believe X means incorrect - not corresponding to sequence in database
6) What do you mean by EMPTY, M, N, X, Y and cells with no data in column G
This was annotation used by the Snyder lab (Heng Zhu) in their original spreadsheet. I believe Y= yes a frameshift, N=no frameshift - so the ORF is in frame, and M=maybe (I proceed with caution when using these plasmids).