OK to use BCA Protein assay with magnetic beads
It is possible to use the BCA Protein assay with the magnetic particles, including the elution step. It is necessary to run a control blank for both the total and bound samples. Dilute the raw material to the same % solids as your sample and continue the assay from there. To calculate the protein, subtract the control blank from each sample accordingly. We also recommend centrifugation or magnetic separation instead of filtering to remove the particles before reading the color.