Can I transform the Yeast Genomic Tiling Collection Assay Ready DNA into bacteria?
No, you should not transform the Yeast Genomic Tiling Collection Assay Ready DNA (cat#YSC5103) into bacteria for several reasons which include:
1. The average insert size in this library is approximately 10kb which is extremely large for transformation and will severely reduce efficiency.
2. The vector (pGP564) is pBR322-based (not pUC-based), so the DNA yield from a plasmid prep from bacteria would be very low.
The amount of plasmid DNA provided in the kit is enough for 10 transformations into yeast for screening purposes only.