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I think I know the best promoter for my cells of interest. Do I still need to evaluate promoter activity with the SMARTchoice shRNA Promoter Selection Plate?
Promoter activity varies in different cell lines and cell types. We have demonstrated that this may affect the expression of the shRNA and the level of gene silencing that can be achieved. If you have previous data and/or knowledge that a particular promoter is active in your cells, then gene-specific shRNAs can be ordered without testing the SMARTchoice shRNA Promoter Selection Plate. If you are not sure which promoter to choose, then testing your cells with the SMARTchoice Promoter Selection Plate prior to ordering gene-specific shRNAs as lentiviral particles can potentially save you a great deal of time and money.