Are there suggestions for sites to digest out puromycin from pGIPZ?

There really isn’t a good way to digest ONLY puromycin out of the pGIPZ vector. However, you can digest IRES-puromycin out using NotI and HpaI. IRES is annotated from 4114-4689 Puromycin is annotated from 4696-5292 We have not tested this in house so we cannot guarantee it to work.