Can RPMI media be substituted for the DMEM media listed in the culturing protocol for the HEK293T cells?
We do not recommend that you use the RPMI media unless for some
experimental reason you have to use it. The two medias do not contain
the same components and our HEK293T cells are
acclimated to the DMEM media listed in the culturing protocol in the
Trans-Lentiviral Packaging System manual.