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I have read that shorter crRNAs (in the targeting sequence, from the 5′ end) are more specific. Can I order shorter crRNAs with less than 20 nucleotides of target sequence?
We have validated the Edit-R system, as well as our manufacturing processes, for crRNAs with 20 nucleotides of target sequence. If a researcher wants to test a shorter crRNA (similar to those described in described in Fu, 2014), then a separate custom RNA oligo must be ordered through the custom single-stranded RNA synthesis route, the pricing will be very different, and we will not troubleshoot or support the results.