Do I need to optimize for cell density?

The Accell Delivery Protocol has shown successful results in over 40 cell types at a range of cell densities. Since there is no lipid transfection reagent to optimize, the primary focus of Accell delivery optimization is cell density. Your cell preference and assay requirements should be taken into account when selecting cell densities for Accell delivery, and it is recommended to assess the growth rate of your cells in Accell delivery media prior to carrying out silencing experiments.