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How should I store my synthetic guide RNAs?
RNA oligonucleotides should be stored at -20 °C or -80 °C in a non-frost-free freezer, either as a dried pellet or resuspended in an RNase-free solution buffered to pH 7.4 to help with stability during freeze-thaw cycles. You may use our 10 mM Tris-HCl Buffer pH 7.4 for resuspension. We recommend that RNA oligonucleotides be resuspended to a convenient stock concentration (Synthetic guide RNA resuspension protocol) and stored in small aliquots to avoid multiple freeze-thaw cycles. RNA oligonucleotides should not go through more than five freeze thaw cycles. If degradation is a concern, the integrity of the RNA oligonucleotides can be evaluated on an analytical PAGE gel.