How can I screen for the best siRNA transfection reagent?
The best transfection reagent is one that results in a high level of siRNA delivery with a low level of cell toxicity. The best method for confirming siRNA delivery is to assay knockdown, preferably at the mRNA level, by a positive control siRNA that targets a housekeeping gene for which silencing is well characterized. We recommend DharmaFECT Transfection Reagents for lipid-based transfection of siRNA. There are four specific formulations that are optimized for efficient delivery into various cell types. Click here to see the DharmaFECT protocol and to learn more.