How do I calculate the amount of ug siRNA in each well if I use 6 uL of a 10 uM stock siRNA solution?
There are several steps to completing this calculation. First, calculate how many nmol siRNA are in each well:
a. equation: ? nmol = (6 uL)(10 umol/L)
b. unit conversions: ? nmol = (6 uL)(10 umol/L)(1 L/10exp6 uL)(1000 nmol/umol)
c. answer: ? nmol = 0.06 nmol
Therefore, there is 0.06 nmol of siRNA in each well.
Next, use the molecular weight of siRNA to convert between nmol and ug. If the specific molecular weight is not known, you may use the average molecular weight of siRNA, which is 13,300 g/mol.
a. equation: ? ug = (0.06 nmol)(13,300 g/mol)
b. unit conversions: ? ug = (0.06 nmol)(13,300 g/mol)(mol/10exp9 nmol)(10exp6 ug/g)
c. answer: ? ug = 0.798 ug, or 0.8 ug
Therefore, there is 0.8 ug of siRNA in each well, when 6 uL of a 10 uM siRNA stock solution is used.