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How do I calculate how much tracrRNA and Tris buffer I will need for my crRNA library?
Since crRNA and tracrRNA are used in equimolar amounts, be sure to purchase enough tracrRNA to complete experiments with all gene-targeting and control crRNAs.
For 0.1 nmol crRNA library: 0.1 nmol x 100 genes = 10 nmols of tracrRNA
For 0.25 nmol library: 0.25 nmol x 100 genes = 25 nmols of tracrRNA
For 0.5 nmol library: 0.5 nmol x 100 genes = 50 nmols of tracrRNA