Was HA antibody or antibodies to each ORF used to detect protein to confirm yeast ORFs?
An HA-antibody was used to detect protein to confirm the yeast ORF clones although you could use an antibody to His-6 as well.
For protocols and specifics, see reference:
Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky, EM, Snyder M, Grayhack EJ (2005). Biochemical and genetic analysis of the yeast proteome with a movable ORF collection. Genes and Development19, 2816–2826.