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Will selecting edited cells by treatment with Puromycin or Blasticidin increase editing efficiency?
Puromycin- or Blasticidin-selection can enrich for cells in which editing has occurred and increase % editing; however, the degree of improvement greatly depends on optimized delivery conditions. If the transfection efficiency of the Edit-R components is high, then antibiotic selection will show a modest improvement in editing efficiency. However, if the transfection efficiency is low, antibiotic selection improves editing efficiency to a greater degree.