Does you sell the C. elegans feeders induced by IPTG?
We don't sell them already induced. The reference paper for the collection contains the protocol (below) that was used in the RNAi screening. We don't do any of this work in house so we rely on these detailed protocols generated by the Vidal lab.
The RNAi screen was performed simultaneously in WT N2 C. elegans hermaphrodites and in a lin-35/Rb mutant background. Standard methods were used for culturing worms on nematode growth medium (NGM; Brenner 1974). Screening was performed in 24-well format plates containing NGM supplemented with 50µg/ml ampicillin, 12.5µg/ml tetracycline, and 1.43 mg/ml IPTG. Unseeded feeding plates were kept in the dark for less than a week at 4°C. Before bacterial inoculation, the feeding plates were dried for 2 to 3h in a laminar airflow hood or in a 37°C incubator. RNAi bacterial clones were grown in 96-well format for 24 h at 37°C in 600µl LB with 50µg/ml carbenicillin and 12.5µg/ml tetracycline. Each well of the 24-well format plates was inoculated with 115µl of bacterial culture by using an extendable automated multichannel pipette. The expression of dsRNA was induced overnight in the presence of IPTG (1.43mg/ml), at room temperature and in the dark. After dsRNA induction, three to 10 worms synchronized in the L1-stage were deposited into the wells.