Do the C.elegans ORF clones have a start and stop codon?
No, this allows a researcher to insert a tag at the N- or C- terminus as referenced in Reboul, et al 2003 (page 40 under METHODS):
"None of our C. elegans cloned ORFs has a methionine codon at position one. For historical reasons, and because the expression vectors were set up for amino terminal fusions, we cloned all the ORFs with a TTG codon in place of the initiator ATG methionine. The ORF should be in frame with the preceeding AAA AAA set in the Gateway elements which will allow for NH-terminal fusions following an LR reaction. The STOP codon is eliminated by replacing one nucleotide to allow for 3’ end fusions."
If the customer wants to begin translation at the cognate ATG, they will have to change that “T” residue back to an “A” and if they don’t want 3’ end fusions, the STOP will need to be restored.