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Can I use the pre-designed Edit-R guide RNA from your website for CRISPRa?
The guide RNA designs for CRISPRa are different than the Edit-R guide RNAs for CRISPR-Cas9 knockout experiments. The guide RNA designs for CRISPRa are required to bind upstream of the transcriptional start site and are based on a published CRISPRa algorithm. Pre-designed Edit-R guide RNAs are optimized for functional gene knockout with the Dharmacon algorithm and target the gene’s coding region.