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Can the Edit-R CRISPRa system be used for gene activation in non-mammalian organisms, such as flies or worms?
We have designed the Edit-R Cas9 plasmids and lentiviral particles for mammalian expression (and thus have only tested) in mammalian cells. The guide RNAs are also pre-designed to activate human and mouse genes. Custom guide RNAs could be ordered that target promoter regions of other species, however we cannot predict the efficacy of using Edit-R lentiviral dCas9-VPR particles and Edit-R synthetic guide RNA components, nor can we troubleshoot experiments performed in non-mammalian systems.