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What are the benefits of using two RNAs (tracrRNA and crRNA) compared to a single guide RNA (sgRNA) expressed from a vector?
The main benefit of using Edit-R with 2 synthetic RNAs is that RNAs for multiple targets can be ordered and transfected within a few days. This allows for rapid evaluation of multiple target sites (by testing multiple crRNAs) within a gene or editing multiple genes. CRISPR-Cas9 systems which utilize expressed guide RNAs require individual cloning of each targeting sequence into a vector backbone, which is especially time consuming when editing of several genes and several target sites per gene are required.