Do I need to anneal the siRNA after resuspension?

All catalog siRNAs and any custom siRNA provided with A4, C, in vivo, or in vivo HPLC processing are provided as a duplex. Reannealing is generally not necessary. However, if reannealing is desired, we recommend heating the siRNA to 55-60°C for 5 minutes, pulse centrifuging, and slow cooling to room temperature for 30 minutes.