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    CRISPR Guide RNA

    Lentiviral and synthetic reagents for targeted gene knockout

    Guide RNAs program Cas9 nucleases to cut at a specific genomic location. The design of an effective, functional guide RNA is critical to achieving efficient gene knockout. Take advantage of our algorithm for functional, specific guide RNAs and search our guaranteed predesigned guide RNAs for your gene of interest.

    Learn more about guide RNA products

    • Synthetic crRNA

      Pre-designed or custom synthesized for rapid knockout studies across many genes

    • Lentiviral sgRNA

      Pre-designed or custom sgRNA as glycerol stocks and high-titer purified lentiviral particles for small- and large-scale studies

    • Synthetic sgRNA

      Custom single guide RNA oligos for CRISPR-Cas9 gene editing

    All Edit-R predesigned guide RNA (synthetic crRNA and lentiviral sgRNA) are designed with a validated algorithm to improve the likelihood of functional knockout, not just double-strand breaks (DSB). By assessing functional phenotypes for thousands of designs, then validating our design rules in other assay systems, we have established rules for determining target sites that are more likely to give efficient cleavage and functional knockout with high specificity. See the Supporting Data tab for more information.

    Edit-R synthetic sgRNA and crRNA can be custom designed to any sequence using the CRISPR Design Tool.

    Guide RNAs in the CRISPR-Cas9 system

    In addition to expression of the Cas9 nuclease, the CRISPR-Cas9 system requires a specific RNA moiety to recruit and direct the nuclease activity. These guide RNAs take one of two forms:

    • A long, chemically synthesized trans-activating CRISPR RNA (tracrRNA) plus a chemically synthesized CRISPR RNA (crRNA) designed to cleave the gene target site of interest (Figure 1a)
    • -or-
    • A synthetic or expressed single guide RNA (sgRNA) that consists of both the crRNA and tracrRNA as a single construct (Figure 1b)

    CRISPR Guide RNA Products

    Synthetic Guide RNA

    • Edit-R predesigned crRNA

      Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!

    • Edit-R tracrRNA

      Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.

    • Edit-R Synthetic sgRNA

      Custom synthetic 100-mer single guide RNA, input your own design or use our flexible design tools

    • Edit-R Synthetic Positive crRNA Controls and Detection Primers

      Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

    • Edit-R Synthetic crRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

    • Edit-R crRNA Libraries

      Plated pre-defined collections of popular gene families for arrayed knockout screening

    • Cherry-pick libraries

      Have a favorite gene list? Customize and order plates of predesigned crRNA for knockout studies in your targets of interest

    All-in-one Lentiviral sgRNA Products

    Lentiviral Guide RNA

    Custom Guide RNA Design & Ordering Tools

    • CRISPR Design Tool

      Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.

    HDR Donor Template Design & Ordering Tools

    HDR Donor Template Kits

    Guide RNA Selection Guide

    Determining the most appropriate type of guide RNA for your experiment will depend on your particular experiment or cell type. Take a look at the features in this table to get started on selecting the best guide RNA reagent.

    All Edit-R predesigned guide RNA (synthetic crRNA and lentiviral sgRNA) are designed with a validated algorithm to improve the likelihood of functional knockout, not just double-strand breaks (DSB).