Design a custom guide RNA for over 30 species with an easy-to-use interface and a choice of advanced options
orEnter your own designs for ordering guide RNAsChoose from synthetic single guide RNA (sgRNA), crRNA, or lentiviral sgRNA
Visit the CRISPR Design Tool Workflow page if you need to learn more about designing a custom guide RNA. Otherwise, select an option below to get started!
You may also choose from highly functional predesigned
synthetic crRNA or lentiviral sgRNA to knockout your human or mouse gene target. Simply
search for your gene.
Design, select, and order a CRISPR guide RNA near a genomic modification site of interest for precise CRISPR-Cas9 gene editing using the HDR pathway.
Once you have guide RNA(s) near a desired modification site, you can use the Edit-R HDR Donor Designer to design your DNA donor.
The Dharmacon CRISPR Design Tool allows you to select CRISPR targeting sequences for incorporation into synthetic crRNAs and single guide RNAs (sgRNAs).
See the CRISPR Design Tool User Guide for detailed instructions.
In some cases, no design results are returned using the default parameters. Below are potential causes and suggestions for modifying the default settings to obtain designs:
In some cases, no crRNA design results are returned using the default parameters. Below are examples of why the default settings may return no crRNA designs, and suggestions for obtaining designs:
The CRISPR Design Tool service is experiencing high traffic. Please try again.
The CRISPR Design Tool has timed out while working on your gene. Please try your design again in a few minutes. If timeouts persist, please contact Dharmacon Technical Support.
Please try again in a few minutes. If problem persists, contact Technical Support.