Our CRISPR Design Tool provides an intuitive one-stop location for guide RNA design and ordering. Use the design tool to order guide RNAs for targeted gene knockout or HDR-mediated genome editing.
Visit the CRISPR Design Tool Workflow page if you need to learn more about designing a custom guide RNA. Otherwise, select an option below to get started!
You may also choose highly functional predesigned synthetic crRNA or lentiviral sgRNA to knockout your human or mouse gene target.
The Dharmacon CRISPR Design Tool allows you to select CRISPR targeting sequences for incorporation into synthetic crRNAs and single guide RNAs (sgRNAs).
See the S. pyogenes Cas9 User Guide or the Alternative CRISPR Nuclease User Guide for detailed instructions.
In some cases, no design results are returned using the default parameters. Below are potential causes and suggestions for modifying the default settings to obtain designs:
In some cases, no crRNA design results are returned using the default parameters. Below are examples of why the default settings may return no crRNA designs, and suggestions for obtaining designs:
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