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CRISPR Design Tool Workflow

Online tool to design & order custom guide RNAs

Design and order custom guide RNAs for targeted gene knockout or HDR-mediated genome editing.

  • Rigorous specificity checking identifies designs with potential off-targets
  • Target protein coding, microRNA, or lncRNA genes for over 40 species
  • Synthetic guide RNAs provided in tubes or plates, lentiviral sgRNA as glycerol stock or concentrated lentiviral particles
  • Advanced options enable transcript-specific designs or designs for a novel sequence
  • Design guide RNA for S. pyogenes Cas9, MAD7, and other CRISPR-Cas systems

The flexibility of the CRISPR Design Tool provides an intuitive one-stop location for custom guide RNA design and ordering. Our bioinformatics team is constantly expanding the capabilities of this resource, so click the Feedback link if you don’t see what you need!

Need to design a guide RNA?

  • Choose from over 40 model organisms
  • Support for protein coding, long noncoding, or microRNA genes; simply enter a gene identifier!
  • Design guide RNA for S. pyogenes Cas9, MAD7 (Cas12a), or any other CRISPR effector
  • Target a specific region for optimal HDR knock-in studies
  • Unparalleled specificity checking for reduced off-target cleavage
  • Intuitive interface lets you visualize guide RNA designs along your gene

Already have a guide RNA design?

  • Enter a target sequence(s) to easily order lentiviral or synthetic guide RNAs for use with S. pyogenes Cas9 nuclease
  • Enter guide RNA sequences for CRISPR-Cas systems such as MAD7, Cas12a, Cas13a, or completely novel systems

Looking for functional knockout without a design step?

You may also choose from highly functional predesigned synthetic crRNA or lentiviral sgRNA to knockout your human or mouse gene target. Simply search for your gene.