The CRISPR Design Tool has four options for designing and/or ordering custom guide RNAs. Choose the best option for your experiment and get started!
Input gene ID or gene symbol: design anywhere within gene: This option is to design guide RNAs for knockout of protein coding, microRNA, or lncRNA genes for over 30 different species.
Input gene ID or gene symbol: specific cleavage location for HDR: This enables design of guide RNA in a specific region to facilitate HDR and knock-in studies. Weighted functionality and specificity ranking is provided.
Provide a DNA region for design: For designs in regions outside of defined genes, other model organisms, or to target a very particular genomic region.
Input my own guide RNA sequence: No design assistance needed? Simply enter a target sequence or the entire length of your synthetic crRNA or sgRNA
By selecting the “Advanced options” link prior to initiating the design step, you have the option to select particular transcript(s) to exclude from guide RNA targeting (default is to target all transcripts)
There are also options to adjust default setting for the purpose of opening up design space when few or no results are returned:
- Turn off the specificity check
- Permit target site to lie outside of the coding region (CDS)
- Adjust the GC content of the target sequence
Adding to cart
When the desired designs have been entered or selected, simply choose the appropriate guide RNA product(s) from one or more of the tabbed lists and click the Add to Cart button (See
Products tab for details). If you select Add to Plate for synthetic crRNA or sgRNA, you will be taken to a separate interface to configure your 96-well plate.