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    CRISPR-Cas9 Gene Editing

    Optimized tools for high-confidence genome engineering

    The Dharmacon Edit-R CRISPR-Cas9 platform greatly simplifies the workflow of permanently knocking out genes. Our approach includes guaranteed, predesigned, ready-to-use guide RNAs and enables fast assessment of multiple target sites per gene for multiple genes. We hold a license from the Broad Institute to provide CRISPR reagents for research use.

    Explore our products for successful CRISPR-Cas9 genome engineering

    • CRISPR Guide RNA

      High quality, ready-to-use lentiviral and synthetic reagents to guide Cas9 cleavage

    • Cas9 Nuclease

      Configure the optimal promoter for your cell type to ensure robust Cas9 expression or explore DNA-free options

    • CRISPR Controls and Detection Primers

      Proper controls are essential to assessment of CRISPR-Cas9 genomic editing experiments

    • CRISPR-Cas9 Screening Libraries

      Pooled sgRNA or arrayed crRNA for high-throughput gene editing studies

    • HDR Donor Templates

      Synthetic single-strand DNA oligonucleotide (ssDNA oligo) donors for precise genomic modification with the homology-directed repair (HDR) pathway

     

    CRISPR genome engineering

    Genome engineering has advanced tremendously with the characterization of bacterial and archael CRISPR (clustered regularly interspaced short palindromic repeats) systems and their adapted usage in mammalian cells. The ability to precisely and permanently alter endogenous gene expression through targeted genome editing is a highly effective reverse genetics tool.

    Multiple publications have shown the use of the Edit-R genome engineering products to target and cleave DNA in mammalian cells, thereby permanently disrupting gene expression, demonstrating this a new and exciting set of molecular tools to interrogate gene function.

    Choosing the right products for a CRISPR Experiment

    There are two required components for CRISPR gene editing - a guide RNA and a source of Cas9 nuclease – each of which are available in multiple formats. Depending on your cell type and application, some options will be better than others. Browse our entire suite of products, or take a few minutes to view this video, which walks through a few high-level questions to help direct you to the best components for your experiment.

     

    Selected CRISPR publications using Edit-R products

    See the full list here.

    1. Tan J, Martin SE (2016) Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening. PLoS ONE 11(12): e0168968. doi:10.1371/journal.pone.0168968
    2. He, K., E. Chou, et al. Conjugation and evaluation of triazole-linked single guide RNA for CRISPR-Cas9 gene editing. ChemBioChem. 17, 1809–1812 (2016).doi:10.1002/cbic.201600320
    3. Hultquist, J.F., K. Schumann, et al. A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells. Cell Reports. 17, 1438–1452 (2016). dx.doi.org/10.1016/j.celrep.2016.09.080
    4. Kelley, M.L., Ž. Strezoska, et al. Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing. J. Biotechnol. 233, 74–83 (2016). doi:10.1016/j.jbiotec.2016.06.011
    5. McCaffrey, J., J. Sibert, et al. CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double-strand DNA for whole genome mapping and structural variation analysis. Nucleic Acids Res. 44(2), e11 (2016). doi:10.1093/nar/gkv878
    6. Paix, A., H. Schmidt, et al. Cas9-assisted recombineering in C. elegans: genome editing using in vivo assembly of linear DNAs. Nucleic Acids Res. (2016). doi:10.1093/nar/gkw502 2015
     

    CRISPR-Cas9 Gene Editing Products

    Synthetic Guide RNA

    • Edit-R predesigned crRNA

      Guaranteed to edit your target! Algorithm-optimized crRNA for genome-wide coverage of human, mouse, or rat genes. Modifications for nuclease resistance improve DNA-free editing. Simply search for your gene!

    • Edit-R tracrRNA

      Edit-R trans-activating CRISPR RNA (tracrRNA) is synthetic, HPLC-purified, long RNA required for use with Edit-R crRNA to form the complex that programs Cas9 nuclease. It is modified for nuclease resistance and can be used with modified or unmodified Edit-R crRNA.

    • Edit-R Synthetic sgRNA

      Custom synthetic 100-mer single guide RNA, input your own design or use our flexible design tools

    • Edit-R Synthetic Positive crRNA Controls and Detection Primers

      Species-specific crRNAs targeting well-characterized genes, as well as mismatch detection assay primers, to determine the effectiveness of your gene editing conditions for maximal efficiency.

    • Edit-R Synthetic crRNA Non-targeting Controls

      Non-targeting controls to evaluate cellular responses to CRISPR-Cas9 components in the absence of gene target-specific crRNA.

    • Edit-R crRNA Libraries

      Plated pre-defined collections of popular human and mouse gene families for arrayed knockout screening.

    • Cherry-pick libraries

      Customize and order plates of predesigned crRNA for knockout studies for your targets of interest.

     

    Lentiviral Guide RNA

     

    Vector-based Cas9 Nuclease

     

    DNA-free Cas9 Nuclease

     

    Custom Guide RNA Design & Ordering Tools

    • CRISPR Design Tool

      Place a custom guide RNA order, or design and order your own synthetic sgRNA, crRNA, or lentiviral sgRNA with our easy-to-use interface.

     

    HDR Donor Template Design & Ordering Tools

     

    HDR Donor Template Kits

     

    The breadth of the Dharmacon portfolio supports multiple gene editing workflows; choose the one that suits your experimental system and desired outcome.

    CRISPR-Cas9 Gene Editing Resources

     

    CRISPR Design Tool

    Do you have a favorite guide RNA sequence?

    Do you require design assistance to ensure Cas9 cleavage in a specific region of your target gene?

    Edit-R predesigned crRNA and lentiviral sgRNA provide algorithm-optimized designs for functionality and specificity; but when particular designs or parameters are desired, you can design your own crRNA or sgRNA with our easy-to-use interface.

    The Dharmacon CRISPR Design Tool allows you to design or enter your own CRISPR targeting sequences for incorporation into synthetic crRNAs and single guide RNAs (sgRNAs).

    Visit the CRISPR Design Tool Workflow page if you need to learn more about designing a custom guide RNA or see the CRISPR Design Tool User Guide for detailed instructions.