Synthetic & lentiviral reagents for endogenous gene overexpression
Dharmacon Edit-R CRISPR activation (CRISPRa) reagents provide a straightforward, efficient set of tools to study a gene’s function by overexpression in its native context. Our predesigned reagents for human and mouse genes make it easy!
CRISPR-Cas9 is not only for creating genomic double strand breaks, it can also be used to target promoter regions to activate or inhibit transcription. Using a deactivated or dead Cas9 nuclease fused to transcription activators, like dCas9-VPR, in combination with a guide RNA in either a synthetic or
lentiviral format, the endogenous expression of a gene can be up-regulated by anywhere from 5 to 50,000+ fold!
Explore our CRISPRa products for transcriptional activation
Arrayed collections of synthetic CRISPR RNA for overexpression screening of gene families, druggable, and whole human genome.
Algorithm-designed lentiviral and synthetic reagents specifically target promoter regions of human and mouse genes to induce a gene’s transcriptional activation.
Lentiviral particles, purified plasmid or mRNA that express nuclease-deactivated Cas9 fused to transcriptional activators. When complexed with a guide RNA will trigger an endogenous gene’s expression.
Chemically synthesized SAM tracrRNA for use with synthetic crRNA for fast and easy gene activation in stable cells containing the SAM transcriptional activation system
Pooled or individual crRNA controls for assessment of optimal experimental conditions for gene activation.
Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of gene target-specific crRNA.
Control sgRNAs targeting well-characterized genes to determine the effectiveness of your experimental conditions for maximum activation.
Non-targeting controls to evaluate cellular responses to CRISPRa components in the absence of target-specific sgRNA.
Upload your own gene list to customize and order plates of predesigned crRNA for CRISPRa studies across tens or thousands of genes.
Edit-R CRISPRa products may be combined in several ways to support your particular experimental needs. Whether you’re doing co-transfection or creating a stable dCas9-VPR cell line (recommended), choose from our synthetic RNA, lentiviral particles, or glycerol stock (plasmid) sgRNA, and combine with dCas9-VPR plasmid or lentiviral particles.