serves as a model organism for the study of embryonic development, developmental biology, cell biology, and oncogenesis. Xenopus
resources include clones from the I.M.A.G.E. Consortium as well as Xenopus
Gene Collection (XGC) clones for both Xenopus laevis
and Xenopus tropicalis
Approximately 9000 clones representing almost 7800 unique gene IDs
ORFs are derived directly from the XGC and placed in a Gateway Donor vector, omitting the stop codon, for easy transfer to an expression vector and 3ʹ end-tagging
ORFs were end sequenced to confirm identity; detailed information on their development can be found here: http://www.xenbase.org/reagents/static/orfeome.jsp
XenopusGene Collection (XGC)
I.M.A.G.E. Xenopus cDNA and EST clones
Fully-sequenced clones were sequenced through the coding region of the gene by the I.M.A.G.E. consortium
Partially-sequenced clones, also known as Expressed Sequence Tags (ESTs), have been 5’ or 3’ end-sequenced by the I.M.A.G.E. consortium and contain all or part of a specific gene
I.M.A.G.E. Consortium website: http://image.hudsonalpha.org/
Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). These clone collections were generated by groups outside of Dharmacon and thus the quality of the collections is largely dependent upon what Dharmacon received from these groups. Specific clone information and plate coordinates were provided to Dharmacon by the suppliers of these clone collections. Dharmacon has not sequence verified each individual clone from these collections. These collections and individual clones are distributed "as is" with no additional product validation or guarantees. Dharmacon has established quality procedures to ensure that individual clones are picked from the identified well in a plate, grown on the correct antibiotic, and are free of phage contamination. Due to the quality of the information provided to Dharmacon, the clone you receive might not match the expected clone. If this occurs, please contact Technical Support.
All clones and plates are provided as glycerol stocks. Individual clones are shipped at room temperature. Plates are shipped on dry ice. Both should be stored at -80°C.